Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Revvity. This protocol. Read & analyse. 96-well culture plateFOR HTRF CERTIFIED READER For more information about HTRF. At Cisbio we provide microplates that help you discover better. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. We would like to show you a description here but the site won’t allow us. BMG LABTECH’s UV-laser not only allows for faster read times in europium TRF assays, but it also greatly enhances performance in terbium-based TRF assays. • HTRF®-Certified Reader. As shown in Fig. PROTOCOL AT A GLANCE. For more information about HTRF. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. High Speed Monochromator offers a typical wavelength accuracy of <0. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. The adapterResults summary for HTRF IP1 standard curve. + 4 µL HTRF pre-mixed antibodies. For a list of HTRF-compatible readers and set-up recommendations, please visit . Plate 25 μL of cells in 96 half-well plate in your appropriate medium. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. It guarantees high level of cAMP accumulation in the cells. Bench top solutions for cell based assays. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Read & analyse. Even if not listed, some readers may be compatible with HTRF technology. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Read & analyse. Revvity. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. It can also be further streamlined toFOR HTRF CERTIFIED READER. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. culture plate. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. This protocol enables the cells' viability and confluence to be monitored. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersHTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. Make sure the setup for Eu 3+ Cryptate is used. overnight at 4 C [Prostaglandin E2] pg/ml. Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. Read & analyse. Incubate overnight at RT. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Its. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Microplate readers are only certified if they. But sometimes these parameters are not sufficient enough. 25,000 human Jurkat cells were plated in 96 well plates. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. This work reports 25 small-molecule binders of the m 6 A-reader domain of YTHDC1. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. The emission of light by the acceptor will be proportional to the level of interaction. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Table 1. The kit includes everything you need: reagents, buffers and a microplate. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. For more information about HTRF. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. . Read & analyse. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . 41 mg/ml) was obtained from Thermo Fisher Scientific. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The plate was covered and incubated for two hours at room temperature. It can also be further streamlined to a one-. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Remove the plate seal and read on an HTRF compatible reader. The ratio for each well must be calculated individually. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. For more information about HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. The FLUOstar Omega is the ideal plate reader for life science applications. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. This protocol gives the cells viability and confluence to be monitored. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. FOR HTRF CERTIFIED READER. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Incubate 24 hours at RT. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. 5 nm filter (PerkinElmer 2100-5110) were used. We recommend using a white plate for time-resolved fluorescence assays and other assays where a lower signal is to be expected, to reduce the signal from background autofluorescence. It can also be further streamlinedFOR HTRF CERTIFIED READER. * For HTRF microplate recommendations, please visit binding of the fluorescent ligand to the receptor, TR-FRET occurs and is measured with an HTRF-certified microplate reader. * For HTRF microplate recommendations, please visit . 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. Conclusion. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF-specific. Your lab might even have one already. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. HTRF is compatible with a large range of available microplate readers. For more information about HTRF. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. FOR HTRF CERTIFIED READER For. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. There are multiple factors to consider including: detection type, additional. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. The detection antibody is incubated for 1. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. NC1656382. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The plate was covered both incubated for one hour at room temperature. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. HTRF Setup. For more. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. 5. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. FOR HTRF CERTIFIED READER For more information about HTRF. Incubate 4h at RT. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. The ratio between control lysate signal / non-specific signal should be greater than 2. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Optimized instrument settings for the HTRF camps HiRange assay. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. Incubate 4h at RT. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. PURCHASE SEPARATELY . The plate is set up for reading and images are acquired according to specified parameters. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For instance, when assay volumes are. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. The mean and standard deviation. FOR HTRF CERTIFIED READER For more. Dedicated UV-laser excitation. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. It enables you to gain insights you couldn’t achieve with detection measurements alone. For the standard curve described, all wells of the microplate are read. Make sure the setup for Tb Cryptate is used. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. FOR HTRF CERTIFIED READER . • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. Incubate 4h at RT. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. For more ® information about HTRF compatible readers and for set-up recommendations, please. VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. Download Setup. For more information about HTRF ®Excitation range. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Live Viewer for cell-based assays. Other technologies such as imaging. Make sure the setup for Tb Cryptate is used. Make sure the setup for Tb Cryptate is used. The controls are equivalent to the upper and lower limit of. Plate 25 μL of cells in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Incubate overnight at RT. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Not for use in diagnostic procedures. For more information about HTRF ®• HTRF®-Certified Reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for 21 CFR Part 11. Highly compatible plates for HTRF. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. For more information about HTRF. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Remove the plate seal and read on an HTRF compatible reader. MATERIALS PROVIDED: KIT . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. culture plate. Read & analyse. To perform the assay, use white plate only. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in. This protocol, HTS designed, allows miniaturization while. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). PURCHASE SEPARATELY . It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Smallest and most affordable multimode plate reader in the industry. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Incubate at room temperature. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator. FOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. They can be used in a no-transfer protocol with suspension cells or with. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. 5 EXAMPLE OF MAP PLATE . FOR HTRF CERTIFIED. For more information about HTRF ®Seal the plate and incubate for Overnight. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. . Assay setup for a 384-well low-volume plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Incubate 4h at RT. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. NON-SPECIFIC BINDING TOTAL BINDINGCover the plate with a plate sealer. Its. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Customer Transformation Hub. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Customer Transformation Hub. For more information. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. For more information about HTRF. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. Specialized filters allow for IR fluorescence. Conclusion. VICTOR Nivo Multimode Plate Reader. Assay detection requires a microplate reader with. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Neocarzinostatin dose-response on Jurkat cells. For more information. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. For more. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. Alpha Technology: The VICTOR Nivo system. Compared to ELISA technology, MSD assays: Require far less sample. Read & analyse. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER For. As shown in Fig. 0. + 4 µL HTRF ® pre-mixed antibo dies 2. Incubate 4h at RT. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. This protocol, HTS designed, allows miniaturization while. DLReady™ certified luminometer plate readers. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. 5. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). FOR HTRF CERTIFIED READER. The plate is set up for reading and images are acquired according to specified parameters. View Brochure. • HTRF®-Certified Reader **. These plates can be read on all HTRF certified readers, thus making their implementation in. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. 1. According to the manufacturers protocol, this protein assay is linear in the range of 0. Revvity. CLARIOstar is equipped with a specific optical device which enables the measurement of. 96-well culture plate• HTRF®-Certified Reader. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. FOR HTRF CERTIFIED. 4 mg/ml. Excitation lasers significantly improve performance and lower. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. This protocol gives the cells viability and confluence to be monitored. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. FOR HTRF CERTIFIED READER. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. The Negative control is used to check the non-specific signal. These plates can be read on all HTRF certified readers, thus making their implementation in. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. This protocol gives the cells viability and confluence to be monitored. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. 1) . The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. For more information about HTRF. 5% and an increased Z’ value of 0. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere.